ndufs3 (Proteintech)
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93
Structured Review
Proteintech
ndufs3
Ndufs3, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/15066+1+ap/NDUFS3+Antibody/pmc12846758-356-79-81
Average 93 stars, based on 45 article reviews
Ndufs3, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/15066+1+ap/NDUFS3+Antibody/pmc12846758-356-79-81
Average 93 stars, based on 45 article reviews
ndufs3 - by Bioz Stars,
2026-09
93/100 stars
Images
1) Product Images from "Microvascular endothelial metabolic dysfunction drives cerebral edema through bioenergetic failure after ischemia-reperfusion"
Article Title: Microvascular endothelial metabolic dysfunction drives cerebral edema through bioenergetic failure after ischemia-reperfusion
Journal: Theranostics
doi: 10.7150/thno.127083
Figure Legend Snippet: DDIT4 facilitates the ubiquitination of NDUFS3 resulted in mitochondrial dysfunction. A, Mass spectrometry analysis of proteins pulled down by anti-DDIT4 antibodies identified potential DDIT4-interacting proteins. B, Volcano plot of differentially expressed proteins between mitochondria isolated from cerebral microvascular tissues before and after ischemia-reperfusion. C, Overlapping analysis of differential proteins in mitochondrial proteomic and interacting proteins of DDIT4. D, Heat map showed the expression of 11 overlapping proteins in mitochondrial proteomic. E, Co-immunoprecipitation of NDUFS3 (left) or DDIT4 (right) in ECs (IgG as a control antibody). F, Western blotting of NDUFS3 in indicated cells. Endothelial cell was treated with MG132 6h for harvest. And the quantification of protein was shown in below (n = 6 per group). G, Western blotting of NDUFS3 in cerebral microvascular tissues from conditional DDIT4 knockdown mice. The quantification of protein was shown in below (n = 6 per group). H, Co-IP of NDUFS3 and then western blotted with anti-ubiquitin in DDIT4 overexpression ECs. I, Representative images of staining with mitoSOX (red) and live cell nuclear stain hoechst33342 (blue). J, Phospho-MLKL aggregation in indicated cells. K, IntDen/cell measured using ImageJ (n = 6 per group). L, p-MLKL aggregates/nucleus quantified using Fiji (n = 6 per group). M, The OCR was measured in indicated cells. Data are mean ± SEM.
Techniques Used: Ubiquitin Proteomics, Mass Spectrometry, Isolation, Expressing, Immunoprecipitation, Control, Western Blot, Knockdown, Co-Immunoprecipitation Assay, Over Expression, Staining
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other:Article Title: Benign prostatic hyperplasia nodules in patients treated with celecoxib and/or finasteride have reduced levels of NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, a mitochondrial protein essential for efficient function of the electron transport chain Article Snippet: NDUFS3 , 15066-1-AP , Immunohistochemistry:Article Title: Antihypertensive Drug Amlodipine Besylate Shows Potential in Alleviating Diabetic Peripheral Neuropathy Article Snippet: .. Antibodies used for immunohistochemistry and western blotting Antibody name Company and catalog number anti-p-AMPK CST, 4185S anti-AMPK CST, 2532S anti-SIRT1 Abcam, ab110304 anti-PGC-1α Abcam, ab54481 anti-COXIV Proteintech, 11242-1-AP anti-NDUFS3 Proteintech, Article Title: Antihypertensive drug amlodipine besylate shows potential in alleviating diabetic peripheral neuropathy. Article Snippet: Schematic summary of the mechanism underlying the amelioration of AB on DPN in mice.. AB as a GPR40 agonist ameliorated DPN-like pathology in both T1DM and T2DM mice.. AB suppressed inflammation by regulating GPR40/β-arrestin2/NLRP3 pathway in sciatic nerves, improved mitochondrial dysfunction through GPR40/LKB1/AMPK/SIRT1/PGC-1α pathway in sciatic nerves and DRG tissues. Western Blot:Article Title: Antihypertensive Drug Amlodipine Besylate Shows Potential in Alleviating Diabetic Peripheral Neuropathy Article Snippet: .. Antibodies used for immunohistochemistry and western blotting Antibody name Company and catalog number anti-p-AMPK CST, 4185S anti-AMPK CST, 2532S anti-SIRT1 Abcam, ab110304 anti-PGC-1α Abcam, ab54481 anti-COXIV Proteintech, 11242-1-AP anti-NDUFS3 Proteintech, Article Title: Antihypertensive drug amlodipine besylate shows potential in alleviating diabetic peripheral neuropathy. Article Snippet: Schematic summary of the mechanism underlying the amelioration of AB on DPN in mice.. AB as a GPR40 agonist ameliorated DPN-like pathology in both T1DM and T2DM mice.. AB suppressed inflammation by regulating GPR40/β-arrestin2/NLRP3 pathway in sciatic nerves, improved mitochondrial dysfunction through GPR40/LKB1/AMPK/SIRT1/PGC-1α pathway in sciatic nerves and DRG tissues. |